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A segment corresponding to amino acids Gln199‐Lys208 of murine IL‐1α cross‐reacts with an antigenic determinant localized in the Z‐line of Drosophila melanogaster myofibrils
Author(s) -
Riparbelli Maria Giovanna,
Callaini Giuliano,
Dallai Romano
Publication year - 1996
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(96)84777-0
Subject(s) - biology , myofibril , drosophila melanogaster , microbiology and biotechnology , immunoelectron microscopy , sarcomere , schneider 2 cells , antibody , myocyte , genetics , gene , biochemistry , rna , rna interference
Summry— Immunofluorescence microscopic observations indicated that a monoclonal antibody, Vmp 18, raised against the peptide 199–208 of murine interleukin 1α, cross‐reacted with an antigenic determinant of Drosophila thorax muscles. Immunoelectron microscopic analysis showed that the gold particles were mainly localized in the Z‐line which is the attachment site of thin filaments from adjacent sarcomeres. On the contrary, the antibody failed to mark the Z‐line in vertebrate skeletal muscle. A Western blot of total protein extract from Drosophila thorax muscles bound a protein of 43 kDa. Our observations suggest that the Vmp 18 antibody could contribute to clarify the composition of the Z‐line in insect's flight muscles.