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Platelet plasma membrane in subjects with primary nocturnal enuresis: Effect of desmopressin
Author(s) -
Giorgi Pier Luigi,
Fiorini Rosamaria,
Biraghi Maurizio,
Kantar Ahmad
Publication year - 1996
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(96)84776-9
Subject(s) - membrane fluidity , desmopressin , membrane , platelet , endocrinology , fluorescence anisotropy , medicine , enuresis , biophysics , biology , biochemistry , urology
Summry— Plasma membrane fluidity of platelets (PLT) obtained from subjects with primary nocturnal enuresis (PNE) and healthy controls was investigated before and after addition of desmopressin (DDAVP). Membrane fluidity was studied by measuring steadystate fluorescence anisotropy of 1‐(4‐trimethylammoniumphenyl)‐6‐phenyl‐1, 3, 5‐hexatriene incorporated into PLT plasma membrane. Our results show an increase in membrane fluidity at the surface level of PLT from subjects with PNE. Moreover, the addition of DDAVP induces a stable and significant decrease of membrane fluidity in both groups. These results suggest alterations of the lipid order in the exterior part of the PLT plasma membrane from patients with PNE.