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Heterogeneous localization of epitopes along axonemes of mammalian cilia
Author(s) -
Mencarelli Caterina,
Tiezzi Antonio,
Ruggiero Paolo,
Contorni Mario,
Massone Annalisa,
Moscatelli Alessandra,
Pallini Vitaliano
Publication year - 1995
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(96)81306-2
Subject(s) - biology , cilium , epitope , microbiology and biotechnology , intraflagellar transport , flagellum , genetics , antigen , gene
Summary— The specificity of four monoclonal antibodies, raised against mammalian ciliary axonemes, was determined by both immunofluorescence and immunoblot experiments. Three antibodies reacted with epitopes which are differentially located along axonemal length. Among these, antibody 3.12 recognized an epitope common to different dynein heavy chains, reacted only with tracheal cilia and specifically stained the proximal portion of the ciliary axoneme.