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A SEM analysis of DMSO treated hydra polyps
Author(s) -
Bolzer Andreas,
Melzer Roland R.,
Bosch Thomas C.G
Publication year - 1994
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(94)90059-0
Subject(s) - lernaean hydra , intracellular , biology , microbiology and biotechnology , cell , cnidocyte , morphology (biology) , extracellular , epithelium , biophysics , biochemistry , genetics , ecology , coral , cnidaria
Summary— Scanning electron microscopy revealed that exposure of hydra polyps to DMSO at concentrations used for permeabilizing tissue results in striking changes in epithelial cell morphology. Epithelial cells from treated polyps rounded up in shape and formed numerous large blebs at the cell surface. Along the borders of epithelial cells numerous small projections became detectable. The DMSO‐induced changes at the cell surface corresponded to drastic changes in the intracellular organization. No evidence could be found for DMSO induced opening of cell junctions and/or opening of the interstitial space. The results demonstrate that DMSO affects the morphology and intracellular organization of hydra epithelial cells. Thus, caution is necessary in interpreting cell behavior in DMSO treated tissue.