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Organization and composition of the striated roots supporting the Golgi apparatus, the so‐called parabasal apparatus, in parabasalid flagellates
Author(s) -
Brugerolle Guy,
Viscogliosi Eric
Publication year - 1994
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(94)90010-8
Subject(s) - biology , immunogold labelling , flagellum , basal body , golgi apparatus , immunofluorescence , microbiology and biotechnology , protozoa , antibody , bacteria , genetics , endoplasmic reticulum
Summary— In parabasalid flagellates, trichomonads and hypermastigids, the stack of cisternae of the Golgi apparatus are supported by striated roots attached to the basal bodies of flagella forming the so‐called parabasal apparatus. Monoclonal antibodies raised for several trichomonad species, Monocercomonas, Trichomonas and Tetratrichomonas , label the parabasal fibre in immunofluorescence or immunogold staining and protein bands in immunoblotting. Several antibodies cross‐react between trichomonad species, and one of them labels the homologous parabasal fibre in the hypermastigids: Trichonympha, Joenia, Pseudotrichonympha and Holomastigotoides . Considering the molecular mass range of the labelled proteins (100–135 kDa) and the lack of antibody cross‐reactivity with the striated root proteins (centrin, assemblin, kinetodesmal protein, ciliary root proteins of epithelial ciliated cells) of other organisms, these proteins recognized by these antibodies seem to represent a new class of protein forming striated roots. The occurrence and significance of parabasal organization in eukaryogenesis is discussed.