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The activation of protein kinase A by cyclic AMP is influenced by oestradiol and progesterone in supernatants from the anterior pituitary but not from hypothalamus of the female rat
Author(s) -
Ostrowska Anna
Publication year - 1994
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(94)90004-3
Subject(s) - ovariectomized rat , medicine , endocrinology , protein kinase a , hypothalamus , estrous cycle , anterior pituitary , biology , hormone , steroid , kinase , microbiology and biotechnology
Summary— The activation of protein kinase A (PKA) by cAMP was estimated in supernatant fractions from the hypothalamus (Hyp) and anterior pituitary (AP) of the female rat during the oestrous cycle and in ovariectomized and ovariectomized, ovarian steroid hormone treated animals. In both structures, the largest activation of PKA was found in dioestrus‐2, while the lowest one was in Hyp in dioestrus‐1 and in AP in oestrus. Ovariectomy had no influence on cAMP‐dependent activation of PKA from Hyp and AP. Treatment of ovariectomized rats with 17‐β‐oestradiol (E 2 ), progesterone (P) or both abolished the activation of PKA by cAMP from AP and had no effect on hypothalamic PKA. These results indicate that ovarian steroids act specifically on AP processes via cAMP dependent pathway and regulation of PKA activity.