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Subcellular distribution of aster‐nucleated microtubule length: A more or less mitotic status of cytoplasmic areas during meiosis I of starfish oocytes
Author(s) -
Barakat Hamid,
GeneviereGarrigues AnneMarie,
Schatt Philippe,
Picard André
Publication year - 1994
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(94)90002-7
Subject(s) - prometaphase , advanced spaceborne thermal emission and reflection radiometer , biology , mitosis , microbiology and biotechnology , metaphase , maturation promoting factor , cyclin b , kinetochore , microtubule , meiosis , cell cycle , cyclin dependent kinase 1 , cyclin , cell , genetics , chromosome , physics , satellite , astronomy , gene
Summary— Shortening of aster‐nucleated microtubules is a mitotic feature, which is proportional to the M‐phase promoting factor kinase activity. We have taken advantage of this characteristic to appraise the mitotic status of cytoplasmic territories in polyspermic starfish oocytes. In early prometaphase, asters are small in the nuclear area, where cyclin B accumulated before GVBD, while in the remaining of the oocytes asters are large. In metaphase all asters are small. This coincides with a change in behaviour of cyclin B, whose association with microtubules increases in late prometaphase. We discuss the possibility that uneven distribution of cyclin B‐dependent kinase is a general feature of mitosis and that it allows the cell to build the convenient spindle at the right place.