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Despite its high representation in extrachromosomal circular DNAs from Drosophila embryos, the dodecasatellite does not allow autonomous replication in cultured cells
Author(s) -
Renault Sylvaine,
Degroote Fabienne,
Picard Georges
Publication year - 1993
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(93)90262-d
Subject(s) - biology , extrachromosomal dna , replication (statistics) , drosophila (subgenus) , embryo , microbiology and biotechnology , dna replication , representation (politics) , genetics , computational biology , dna , virology , gene , plasmid , politics , political science , law
Summary— The dodecasatellite is a 11/12 bp tandemly repeated sequence which is overrepresented, with regard to its genomic representation, in extrachromosomal circular DNAs from D melanogaster embryos. Here we show that a bacterial plasmid carrying a cluster of dodecasatellite is not able to replicate efficiently in cultured cells. This observation does not support the hypothesis that the over‐representati results from an autonomous replication of dodecasatellite circular DNA molecules.