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Protein uptake by intestinal macrophages and eosinophilic granulocytes in trout: An in vivo study
Author(s) -
Dorin Dominique,
Martin Patrick,
Sire MarieFrance,
Smal Jean,
Vernier JeanMarie
Publication year - 1993
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(93)90260-l
Subject(s) - heterologous , biology , lamina propria , microbiology and biotechnology , trout , immunology , epithelium , biochemistry , genetics , fishery , gene , fish <actinopterygii>
Summary— A homologous protein, recombinant trout somatotropin (rtST) and its heterologous counterpart, native bovine somatotropin (bST), were administered anally to juvenile rainbow trout ( Oncorhynchus mykiss ). Plasma levels of rtST, determioned by radio‐immunoassay, peaked between 15 and 60 min and remained high until 2 h after administration. Immunogold labelling was used to follow the routes of transfer of rtST and bST, and to observe potential interaction between the hormones and the cells constituting the first line of non‐specific defence, ie the macrophages infiltrated between epithelial cells, or dispersed in the subepithelial lamina propria, and eosinophilic granulocytes (EGCs) of the lamina propria, whose properties have been considered to be similar to mammalian mast cells. Macrophages were immunolabelled for the homologous and heterologous proteins. EGCs took up to the heterologous but not the homologous protein. This finding was confirmed using indirect immunofluorescence assay. EGCs could internalize foreign proteins transferred from the intestinal lumen to the lamina propria.