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Localization and partial characterization of a rat ovarian granulose cell protein with a monoclonal antibody
Author(s) -
Russinova Angelina,
Vassilev Alex,
Davidoff Michail
Publication year - 1993
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(93)90145-5
Subject(s) - biology , monoclonal antibody , microbiology and biotechnology , antibody , immunology
Summry— Hybridoma cell lines were obtained from mouse splenocytes sensitized to granulosa cells collected from rat ovaries after gonadotropin stimulation. A monoclonal antibody (5G5) was obtained which reacted with granulosa cells and showed a positive reaction with serum‐free conditioned medium containing granulosa cell secreted proteins. Immoblotting of the conditioned medium and light‐ and electron‐microscopic immunocytochemistry of rat ovary show that mAb 5G5 is directed against a 59‐kDa protein which is located on the plasma membrane of granulosa cells. Furthermore, the immunoreactivity of the granulosa cells depends both on the degree of follicle development and on the position of the granulosa cells within the follicles. Strong immunoreactivity was observed in the innermost granulosa cell layers, close to the oocyte and the antral cavity. The results obtained show that mAb 5G5 is a useful marker of a 59‐kDa granulosa cell protein which might be of importance for the follicle and the occyte maturation.