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Image and flow cytometry: Companion techniques for adherent and non‐adherent cell analysis and sorting
Author(s) -
Métézeau Philippe
Publication year - 1993
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(93)90123-v
Subject(s) - flow cytometry , cell sorting , sorting , biology , cytometry , confocal , fluorescence microscope , confocal microscopy , fluorescence , confocal laser scanning microscopy , biomedical engineering , microbiology and biotechnology , biophysics , computer science , optics , physics , medicine , programming language
Summary— Flow cytometry (FMC) is an analytical and preparative technique whereas image analysis is only applied to cell analysis. Recently, image analysis has been adapted as a preparative method using a new technique: image cytometry for analysis and sorting (ICAS). FCM and ICAS are complementary. Flow cytometry allows rapid, quantitative and precise study of fluorescence and light scattering in a large number of cells in suspension, while ICAS analyses fewer cells (adherent cells or tissue) on the basis of fluorescence, morphology and size. ICAS can use these criteria to destroy unwanted cells and hence sort selected cells. ICAS can also be used for confocal microscopy and laser surgery.