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Flow cytometry's contribution to the measurement of cell functions
Author(s) -
Maftah Abderrahman,
Huet Olivier,
Gallet PaulFrançois,
Ratinaud MarieHélène
Publication year - 1993
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(93)90119-y
Subject(s) - flow cytometry , biology , endocytosis , cell sorting , sorting , cell , microbiology and biotechnology , flow (mathematics) , biological system , biophysics , biochemistry , computer science , physics , mechanics , programming language
Summary— Flow cytometry has important advantages over conventional techniques. It is rapid, highly sensitive and allows multi‐parametric analysis and cell sorting. Potential exists for the measurement of many cell functions by flow cytometry. The technique can be used to determine cell viability, intracellular calcium and pH, membrane potential, enzyme activities, membrane fluidity and endocytosis. Numerous examples are given on the applications of flow cytometry for cell functions measurements in the fundamental and biomedical fields.