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Real‐time observation of microtubules attached to microtubule organizing centers in vitro *
Author(s) -
Cibert Christian,
Huitorel Philippe
Publication year - 1992
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(92)90438-7
Subject(s) - microtubule , kinetochore , biology , astral microtubules , microbiology and biotechnology , mitosis , advanced spaceborne thermal emission and reflection radiometer , spindle apparatus , biophysics , cell division , cell , physics , genetics , chromosome , satellite , astronomy , gene
Summary— The dynamics and organization of microtubules associated with axonemes and kinetochores in vitro were visualized using video microscopy techniques. Microtubules attached either at the ends of axonemes or to mitotic chromosomes behave accordining to dynamic instability in our conditions. Microtubules attached to kinetochores showed lower rates of elongation and shortening than those nucleated by axonemes in the same conditions. In addition, elementary bundles of microtubules appeared spontaneously in association with kinetochores, with microtubules elongating along previously attached microtubules at even lower rates. Such side interactions, either spontaneous or stabilized by factors such as MAPs, might affect microtubule dynamics directly.