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Synthesis and secretion by mouse 3T3 cells of a inhibitor of cell growth (mIGFBP‐3): Correlation with cell proliferation
Author(s) -
Liu Li,
Blat Christiane,
Harel Louise
Publication year - 1992
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(92)90204-e
Subject(s) - dna synthesis , biology , stimulation , incubation , 3t3 cells , growth factor , cell growth , platelet derived growth factor receptor , secretion , basic fibroblast growth factor , cell culture , microbiology and biotechnology , fibroblast growth factor , cell , dna , endocrinology , biochemistry , receptor , transfection , genetics
Summary— Our results show that stimulation by serum of dense cultures of 3T3 cells rapidly induced increased synthesis of a growth inhibitor (mIGFBP‐3) capable of binding IGF. mIGFBP‐3 was secreted by stimulated cells immediately after its synthesis, and accumulated in the medium. Accumulation of mIGFBP‐3 in the medium increased, as a function of growth factor (bFGF, PDGF, insulin) concentrations and time. bFGF was the best stimulatory factor for both DNA synthesis and accumulation of mIGFBP‐3 in the first 24 h of incubation. DNA synthesis was arrested after 48 h of incubation with bFGF when accumulation of mIGFBP‐3 was maximal. Since we showed that mIGFBP‐3 is able to inhibit bFGF stimulation of DNA synthesis in mouse fibroblasts, it is possible that the accumulation of mIGFBP‐3 induces a feedback regulation of cell growth.