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Alterations in erythrocyte membrane fluidity in children with trisomy 21: a fluorescence study
Author(s) -
Kantar A.,
Giorgi PL,
Curatola G.,
Fiorini R.
Publication year - 1992
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(92)90133-l
Subject(s) - membrane fluidity , fluorescence , trisomy , membrane , fluorescence anisotropy , biology , biophysics , biochemistry , genetics , optics , physics
Summary— Membrane fluidity of erythrocytes obtained from 15 children with trisomy 21 and 20 healthy controls were studied by measuring steady‐state fluorescence anisotropy and fluorescence lifetime of 1,6‐diphenyl‐1,3,5‐hexatriene (DPH) and 1‐(4‐trimethylammoniumphenyl)‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH) incorporated in hemoglobin‐free erythrocyte membranes. Our results demonstrate a significant decrease in DPH fluorescence anisotropy and a significant increase in TMA‐DPH fluorescence anistropy in erythrocytes from subjects with trisomy 21. No significant differences between the two groups were observed in the fluorescence lifetime of DPH and TMA‐DPH. These data suggest an increase in membrane fluidity in the interior part of the membrane and a decrease in fluidity at the lipid‐water interface region. This could be in part attributed to an increased oxidative damage in trisomy 21.