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In vitro effect of fungal cyclodepsipeptides on leukemic cells: study of destruxins A, B and E
Author(s) -
Odier Franc¸oise,
Vey Alain,
Bureau JP
Publication year - 1992
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(92)90037-2
Subject(s) - biology , in vitro , microbiology and biotechnology , biochemistry
Summary— The activities of three mycotoxins isolated from the hyphomycete Metarhizium anisopliae : destruxin A, B, and E (DA, DB and DE) are described and compared in vitro on leukemic cells. Their antitumor effect was investigated by flow cytometry on growth, cell viability and cell cycle perturbation 48 h after destruxin exposure. Against P388 leukemic cells, DE displayed greater antiproliferative activity than DA and DB. The minimum concentration required to inhibit 50% of cell proliferation is 0.33 μg/ml for DE, 11.7 μg/ml for DA and 9.4 μg/ml for DB. Cell cycle modifications were only observed with DE at 50 and 10 μg/ml and consisted in an accumulation of the cells in GO/1 phase. DA and DB did not modify the number of cells in G0/1 of the cell cycle. Nevertheless a decrease in the number of cells in G2 + M phase was induced by the three destruxins.