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Calcium‐induced acetylcholine release and intramembrane particle occurrence in proteoliposomes equipped with mediatophore
Author(s) -
Brochier Guy,
GulikKrzywicki Tadeusz,
Lesbats Bernard,
Dedieu JeanClaude,
Israel Maurice
Publication year - 1992
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(92)90029-z
Subject(s) - acetylcholine , calcium , electric organ , biology , torpedo , biophysics , microbiology and biotechnology , endogeny , acetylcholine receptor , biochemistry , endocrinology , medicine , receptor
Summary— Proteoliposomes obtained from the mediatophore, a purified Torpedo electric organ nerve terminals protein, and endogenous lipids were used for a study of calcium‐induced release of acetylcholine and freeze‐fracture electron microscopy. Large intramembrane particles were induced by the influx of calcium into proteoliposomes, as previously observed for synaptosomes or stimulated electric organ nerve terminals. The involvement of mediatophore in a calcium dependent acetylcholine translocation seems therefore to be related to the occurrence of a category of intramembrane particles in the course of the release process.