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Immunogold labelling of arabinoxylans in the plant cell walls of normal and bm3 mutant maize
Author(s) -
Barry Patrick,
Prensier Gérard,
Grenet Elisabeth
Publication year - 1991
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(91)90275-r
Subject(s) - immunogold labelling , biology , mutant , labelling , cell wall , polyclonal antibodies , parenchyma , cytoplasm , biochemistry , botany , ultrastructure , gene , antibody , genetics
Summary— Polyclonal antibodies directed against α, l ‐1.2‐arabinofuranosyl poly‐β, d ‐1.4‐xylopyranosyl (degree of polymerization 130) have been raised from rabbits. The immunogold labelling in transmission electron microscopy (TEM) evidenced the arabinoxylans of the plant cell walls. Comparison between the stems of normal and mutant bm3 maize demonstrated a greater accessibility of arabinoxylans in the walls of the mutant maize. The method, specific and swift, allows us to specify the repartition in the different parts of the stem: sclerenchyma, fibers, parenchyma.