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Structure of the basement membrane of corneal epithelium: quick‐freeze, deep‐etch comparative study of networks deposited in culture and during development
Author(s) -
Barge Annie,
Ruggiero Florence,
Garrone Robert
Publication year - 1991
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(91)90088-5
Subject(s) - basement membrane , biology , epithelium , type iv collagen , microbiology and biotechnology , extracellular matrix , membrane , corneal epithelium , electron microscope , matrix (chemical analysis) , fibril , anatomy , basement , biophysics , laminin , materials science , optics , biochemistry , composite material , genetics , physics , civil engineering , engineering
Summary— The basement membranes elaborated by corneal epithelium in the chick embryo and in culture conditions have been studied by quick‐freezing and deep‐etching methods. Electron microscope observations of en face unidirectional platinum shadow castings revealed a polygonal network comparable to the type IV collagen network described in human amniotic basement membrane and EHS mouse tumor matrix [22]. The material deposited in culture contained type IV collagen, as demonstrated by immunofluorescence labeling using anti‐type IV collagen antibodies and formed delicate networks. Fine filaments and granules composing this network were interpreted respectively as linear and globular NCI domains of the type IV collagen molecule. These loose networks were considered as first steps in basement membrane assembly. Staggered superimposition of comparable networks could lead to the dense network organization as observed for basement membranes in situ . These observations showed that the basement membrane of the chick embryo corneal epithelium is also organized in a complex polygonal framework that is preserved even when secreted in cultrue conditions.