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Substance P and calbindin D‐28k‐immunoreactivity in primary sensory neurons of chick embryos: differential neuronal birthdates and transient co‐localization
Author(s) -
Duc Christophe,
BarakatWalter Ibtissam,
Droz Bernard
Publication year - 1991
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(91)90053-p
Subject(s) - biology , neuroblast , embryo , calbindin , vitamin d dependent calcium binding protein , embryonic stem cell , sensory system , microbiology and biotechnology , medicine , neuron , neuroscience , endocrinology , immunohistochemistry , anatomy , neurogenesis , immunology , biochemistry , gene
Summary— During the ontogenesis of dorsal root ganglia (DRG), the immunoreactivity to substance P (SP) and calbindin D‐28k (CaBP) appears in chickens at embryonic day 5 (E5) and E10 respectively. To establish the birthdates of primary sensory neurons expressing SP or CaBP, chick embryos were given repetitive intra‐amniotic injections of [ H H]‐thymidine. The neuroblasts giving rise to SP‐expressing neurons were labeled up to E6 while those generating CaBP‐immunoreactive neurons stopped to incorporate [ 3 H]‐thymidine before E5.5. This finding indicates that neurons exhibiting distinct phenotypes may originate from neuroblasts which arrest to proliferate at close but distinct stages of development. To determine whether SP and CaBP are co‐expressed or not in DRG neurons, chick embryos at E12, E18, and chickens two weeks after hatching were perfused and fixed to detect simultaneously SP‐ and CaBP‐immunoreactivity in DRG sections. The results showed that SP and CaBP were transiently co‐expressed by a subset of neurons at E12. Later, however, the SP‐immunoreactivity was gradually lost by these ganglion cells, so that the SP‐ and CaBP‐immunoreaction defined two distinct neuronal subpopulations after hatching. In conclusion, most CaBP‐immunoreactive DRG cells derive from a subset of neurons in which SP and CaBP are transiently co‐localized.