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Ultrastructural features of soybean somatic cells at the beginning of an organogenic process: toward a new concept *
Author(s) -
Fournier Dominique,
Bonnelle Christine,
Tourte Yves
Publication year - 1991
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(91)90014-e
Subject(s) - biology , somatic cell , ultrastructure , microbiology and biotechnology , cytoplasm , protoplast , nucleus , regeneration (biology) , chromatin , mitochondrion , lineage (genetic) , botany , meiocyte , genetics , meiosis , gene
Summary— Histological and ultrastructural observations of soybean protoplast‐derived calli allowed establishment of the very early cellular reorganization that initiates a proliferating process at the beginning of regeneration. These cytological events appeared more clearly in an inductive medium containing 2.4 D. The cells that participate in the somatic regeneration are characterized by special features that chiefly affect the nucleus and mitochondria: an increase of nucleo‐cytoplasmic exchange by means of deep invaginations of the nuclear envelope, the occurrence of specific attachments of chromatin dense masses at the bottom of these invaginations and the disappearance of some mitochondria while a great number of them proliferates around the nucleus. All these events are comparable to the cytological reorganization affecting the female gametic lineage that generally occurs during the female gametophytic phase in sexual plant reproduction.