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Vasopressin‐induced changes in receptor‐mediated endocytosis of asialoglycoprotein in rat hepatocytes
Author(s) -
GilFalgon Sophie,
Lamaze Christophe,
Feger Jeanne
Publication year - 1991
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(91)90007-a
Subject(s) - endocytosis , internalization , biology , intracellular , diacylglycerol kinase , microbiology and biotechnology , receptor mediated endocytosis , vasopressin , asialoglycoprotein receptor , second messenger system , receptor , vasopressin receptor , endosome , biochemistry , hepatocyte , signal transduction , protein kinase c , endocrinology , antagonist , in vitro
Summary— The ability of second messengers to modulate receptor‐mediated endocytosis was studied on isolated rat hepatocytes. A 20‐min preincubation with vasopressin was used as a modulation. We observed a 20% inactivation of both surface and intracellular receptors, with no change in the affinity of those remaining active. The internalization and dissociation of a synchronous wave of ligand was not affected, but its degradation was partially inhibited. Our observations suggest that second messengers such as intracellular calcium and diacylglycerol play a complex role in the intracellular trafficking associated with endocytosis.