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Microcalorimetric investigations on human leukemia cells — Molt 4
Author(s) -
Nittinger Jürgen,
TejmarKolar Liana,
Fürst Peter
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90370-i
Subject(s) - isothermal microcalorimetry , biology , glycolysis , biochemistry , cell culture , cell , biophysics , microbiology and biotechnology , metabolism , thermodynamics , physics , genetics , enthalpy
Summary— Heat production by leukemia cells Molt 4, growing in suspensions with 1 and 3 × 10 5 cells/ml for 4 days, was studied by microcalorimetry. Heat production rates were related to cell growth, glucose consumption, lactate production and cellular ATP‐content. The results show that the time course of heat dissipation is dependent on initial cell number. However, observed thermal power maxima were fairly identical in all experiments. Heat production rates per cell were similar during the initial phase of the study independently of initial cell number, while higher cell densities resulted in significantly lower rate of heat production. Glycolytic conversion of glucose into lactate is nearly stoichiometric. Our results indicate a relationship between heat production and amounts of glucose and lactate in the medium. ATP concentration in cells decreased after 24 hours of culture.