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Candida albicans — adriamycin interactions: ultrastructural and spectrofluorometric study of whole yeasts and spheroplasts
Author(s) -
Bobichon Hélène,
Bussy Véronique,
Angiboust JeanFrançois,
Manfait Michel,
Bouchet Philippe,
Jardillier JeanClaude
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90360-f
Subject(s) - candida albicans , spheroplast , biology , ultrastructure , nucleolus , candida tropicalis , in vitro , microbiology and biotechnology , cell , metabolite , biochemistry , gene , cytoplasm , anatomy , escherichia coli
Summary— The occurrence of candidiasis in cancer patients who undergo chemotherapy requires the interrelation of Candida albicans and the antimitotic drug Adriamycin (ADM) which is well known as an intercalating agent. The whole yeasts were not affected by 2 h of contact with the drug at 10 −4 M neither for their growth curve nor for their ultrastructure, despite the presence of free ADM on their surface. Spheroplasts displayed a delay in their growth and exhibited altered nucleoli with segregation of their granular and fibrillar components. The modified emission spectrum of ADM, determined by spectrofluorometry, corresponded neither to the free ADM nor to the DNA‐bound drug, but it could be related to a metabolite of the drug. The cell wall appeared to be one of the main sites for ADM resistance of Candida albicans in vitro.