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Low tumorigenicity of canine cells transformed by the human cytomegalovirus
Author(s) -
Yelle Jocelyn,
Lussier Gilles,
Pramatarova Albena,
Hamelin Claude
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90355-7
Subject(s) - biology , human cytomegalovirus , virology , transfection , endonuclease , cytomegalovirus , restriction enzyme , embryo , microbiology and biotechnology , virus , cell culture , antigen , dna , agarose , herpesviridae , immunology , viral disease , genetics
Summary— Dog embryo kidney cells transformed by the human cytomegalovirus (HCMV) were obtained after non‐permissive infection or transfection with viral DNA digested by restriction endonuclease Eco R I. The transformed cells, growing rapidly and showing an unlimited division potential, could use medium with only 2% serum for growth, contained nuclear virus antigens, and formed small colonies (< 0.2 mm) in agarose. From 40 mice inoculated with transformed canine cells, only one eventually developed a tumor. Results indicate that dog cells are immortalized but not tumorigenically transformed by the human cytomegalovirus.