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The stroma‐vascular fraction of rat inguinal and epididymal adipose tissue and the adipoconversion of fat cell precursors in primary culture
Author(s) -
Gre´goire Francine,
Todoroff Germaine,
Hauser Nadine,
Remacle Claude
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90348-7
Subject(s) - adipose tissue , biology , stroma , cell type , stromal vascular fraction , cell , cell culture , lipoprotein lipase , endocrinology , lipid droplet , epididymis , medicine , microbiology and biotechnology , biochemistry , immunology , genetics , immunohistochemistry , botany , sperm
Summary— The stroma‐vascular fraction (SVF) of inguinal and epididymal fat pads of 4 week‐old rats was studied by electron microscopy. Among the various cell types, endothelial cells and preadipocytes were found in both SVF, while mesothelial cells were only detected in the epididymal SVF. The resulting heterogeneity of primary culture and the adipoconversion of the fat cell precursors were studied in a serum‐supplemented medium enriched with insulin (14.5 nM) and exogeneous triglycerides. Despite the heterogeneity of the inoculum, the primary cultures were rather homogeneous, fat cell precursors being the main cell type. Distinctive contaminant fibroblast‐like cells were observed in both cultures, whereas epithelial‐like cells, which correspond most probably to mesothelial cells, were only found in epididymal cultures. Differentiation of fat cell precursors was assessed by the appearance of lipoprotein lipase (LPL) and glycerol‐3‐phosphate dehydrogenase (GPDH). LPL activity was found in the same level in cells of both deposits while GPDH activity was elevated in inguinal vs epididymal derived stroma‐vascular cells. The different adipose conversion pattern of both cultures was confirmed by morphological quantification: the maturation of epididymal fat cell precursors was faster but less extensive. These differences could be related mainly to regional localization rather than to different maturation of the two fat deposits.