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Transfer of immortality by transfection of genomic DNA from SV40 established cell lines into rat embryo fibroblasts
Author(s) -
JacqueminSablon Hélène,
Ganz Leonard,
Feunteun Jean
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90312-q
Subject(s) - biology , transfection , embryo , gene , fibroblast , microbiology and biotechnology , phenotype , immortality , cell culture , dna , genetics
Summary— The cellular immortalization activity of cloned genes can be identified either in a colony‐forming assa of transferred primary rat embryo fibroblasts or in a co‐operation assay together with ras . However the demonstration of immortalization activities carried by cellular genes has not been reported. Here we establish that SV40 early genes integrated in genomic DNAs can be stably transferred into rat embryo fibroblasts and selected via their immortalization activity. Attempts to extend this assay to the identification of dominant genes putatively involved in the immortality of several other immortal post‐crisis or tumor cells have been unsuccessful suggesting that the immortal phenotype can be brought about through different pathways.