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Immunochemical localization and association with spermatozoa of androgen‐regulated proteins of MR 24000 secreted by the mouse epididymis
Author(s) -
Jimenez Clément,
Ghyselinck Norbert B.,
Depeiges Annie,
Dufaure JeanPierre
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90304-l
Subject(s) - epididymis , biology , polyclonal antibodies , antigen , secretory protein , lumen (anatomy) , androgen , duct (anatomy) , microbiology and biotechnology , sperm , secretion , andrology , endocrinology , anatomy , immunology , hormone , genetics , medicine
Summary— A polyclonal monospecific immune serum was raised against androgen‐regulated proteins with Mr 24000 secreted by the mouse caput epididymidis (6). Sections of frozen tissues from the different regions of the epididymis have been studied by indirect immuno‐ fluorescence. Results indicate that the antigens are secretory proteins produced by the epithelial cells of the caput epididymidis, essentially in the medial and distal segments. Accumulation of the antigens was observed in the lumen of the caput and the corpus epididymal duct. Subsequently, their association with the sperm surface occurred and persisted down to the cauda epididymidis.