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In situ separation of nucleolar components by hypotonic treatment of cells
Author(s) -
Hozák Pavel,
Zatsepina Olga V.,
Likovský Zbyněk
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90303-k
Subject(s) - nucleolus , biology , tonicity , in situ , microbiology and biotechnology , biophysics , biochemistry , nucleus , chemistry , organic chemistry
Summary— The main features of the reaction of nucleolar components to a simple hypotonic shock are described. In place of nucleoli, only fibrillar complexes remain. Such preservation provides another morphological proof of the hypothesis of fibrillar complexes as morphological‐functional units in active nucleoli. Moreover, this method can become a good tool for further study of nucleolus‐organizing regions.