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Response of meristem cells to hyperoxygenation
Author(s) -
GiménezAbián M. Inmaculada,
LópezSáez Jorge F.,
Torre Consuelo
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90302-j
Subject(s) - biology , caffeine , meristem , hypoxia (environmental) , oxygen tension , oxygen , biophysics , botany , endocrinology , chemistry , shoot , organic chemistry
Summary— Cycle parameters were estimated in Allium cepa L meristems proliferating under steady state conditions in tap water aerated with either 21 or 100% O 2 and containing 7.9 and 39.0 mg/1 of dissolved oxygen, respectively. Cycle time was slightly but consistently reduced under hyperoxygenation, as shown in synchronous cells labelled as binucleate following a short treatment with 5 mM caffeine. By autoradiography after 3 H‐thymidine treatment followed by microdensitometry of unlabelled interphases it was determined that cellular frequencies at the last third of the S period (S 3 ) were statistically different ( P <0.05) in control and hyperoxygenated conditions. Estimation of absolute timing confirmed that S 3 duration was nearly halved under the increased O 2 tension. Finally, G 1 time was not modified by hyperoxygenation, though this cycle stage lengthened under hypoxia.