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Study of the heterogeneity of a human non‐small cell bronchopulmonary epidermoid primary carcinoma: determination of the DNA content of NSCLCN 6 L 2 cells and of cloned sub‐populations
Author(s) -
Gratas Catherine,
Robillard Nelly,
Roussakis Christos,
Audouin AnneFrançoise,
Verbis JeanFrançois
Publication year - 1990
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(90)90298-h
Subject(s) - biology , ploidy , flow cytometry , cloning (programming) , cell culture , microbiology and biotechnology , dna , population , epidermoid carcinoma , in vitro , genetics , carcinoma , gene , demography , sociology , computer science , programming language
Summary— A continuous cell line, NSCLCN 6 L 2 , was established in vitro from a human bronchopulmonary epidermoid carcinoma and then cloned on agar gel and by selective media. The DNA content of each sub‐population was compared with that of the parent line by flow cytometry. This study showed the heterogeneity of the NSCLCN 6 L 2 line and the possibility of selection by cloning distinct ploidy sub‐populations (group 1: diploid lines; group 2: hypotetraploid lines; group 3: a diploid and tetraploid line); it also allowed the time‐course of the evolving lines to be followed. Correlation of these results with other properties of the different sub‐populations will provide a better understanding of their biological behavior, particularly of their chemosensitivity.