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ULTRASTRUCTURAL LOCALIZATION OF ARGYROPHILIC PROTEINS IN NUCLEOLI OF ZEA MAYS BY TWO SILVER STAINING TECHNIQUES
Author(s) -
MOTTE Patrick,
MOSEN Henri,
BRONCHART Raymond,
DELTOUR Roger
Publication year - 1988
Publication title -
biology of the cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.543
H-Index - 85
eISSN - 1768-322X
pISSN - 0248-4900
DOI - 10.1016/0248-4900(88)90099-8
Subject(s) - staining , nucleolus , biology , silver stain , ultrastructure , zea mays , anatomy , microbiology and biotechnology , biophysics , pathology , nucleus , genetics , agronomy , medicine
Summry— Ag staining was applied on interphasic nucleoli of Zea mays root cells 120h after germination. We applied the two‐step Ag‐NOR staining technique to small root fragments and the one‐step technique to sections of Lowicryl‐embedded tissue. The small‐sized silver grains were mainly located in the dense fibrillar component (DFC). The unstained fibrillar centers (FCs) differed in their proteinic contents from the NOR (which is positively silver stained) and were not the interphasic NOR counterpart.