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Quantification of immunoglobulin A in chorioamniotic membrane of patients with premature rupture of membranes
Author(s) -
Berezoswski A.T.,
Cunha S.P.,
Costa J.C.,
Bacchi C.E.
Publication year - 1994
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/0020-7292(94)90456-1
Subject(s) - medicine , radial immunodiffusion , prom , membrane , antibody , immunoglobulin a , immunoglobulin g , immunology , obstetrics , biochemistry , biology
Objective: The proposal was to study the presence of immunoglobulin A (IgA) in the chorioamniotic membrane of healthy postpartum women with premature rupture of the chorioamniotic membrane (PROM). Method: A single radial immunodiffusion technique was used to quantify the IgA in the chorioamniotic membrane tissues. Results: The level of IgA was approximately 10 times higher in patients whose membranes had been ruptured for > 10 h (24.58 mg/dl). These results were compared with those of a previously published study where the mean of amount of IgA was 2.52 mg/dl in membranes of patients with rupture < 10 h. Our results show that IgA began to rise after 10–15 h following rupture. Conclusion: Although more studies need to be performed our data indicate that the increasing IgA in our patients after 10 h of latency probably represents the beginning of an ascending colonization of bacteria which could be the source of future infection.