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Preparation of human frozen‐thawed seminal specimens using the SpermPrep filtration method: Improvements over the conventional swim‐up method
Author(s) -
Zavos PM
Publication year - 1993
Publication title -
international journal of gynecology and obstetrics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.895
H-Index - 97
eISSN - 1879-3479
pISSN - 0020-7292
DOI - 10.1016/0020-7292(93)90879-2
Subject(s) - medicine , citation , gynecology , information retrieval , library science , computer science
specimen. Main Outcome Measures: Spermatozoa recovered via the SpermPrep filtration and swim-up processes were evaluated for sperm numbers recovered, sperm motility, grade of sperm motility, percentage of morphologically normal spermatozoa, the response of spermatozoa to a hypo-osmotic environment (hypo-osmotic swelling test), and the amount of debris present. Results: Application of the SpermPrep filtration method resulted in recovery of significantly greater numbers of spermatozoa (P < 0.01) than were recovered with the swim-up method (31.1 + 3.2 X lo6 versus 10.2 + 1.8 X lo6 spermatozoa, respectively). This represents a mean recovery of approximately one half (49%) of all spermatozoa applied to the filter, whereas for the swim-up method, it was only 15%. The overall quality of recovered spermatozoa was virtually identical between the two methods (P > 0.05). The percent motile sperm improved by a mean of 18% to 20%, the grade of motility improved by a mean of 0.4 points (scale 0 to 4), the percent of morphologically normal spermatozoa increased by a mean of approximately lo%, the percent of spermatozoa reactive to a hypo-osmotic medium test increased by a mean of approximately 9%, and the debris score decreased by a mean of 0.2 to 0.3 points (scale 0 to 4). Most importantly, the mean total number of motile, morphologically normal spermatozoa after filtration through the SpermPrep column was 20.2 + 1.1 X 106, representing a mean recovery of 73% of the normal spermatozoa originally applied to the column. This was 316% greater than the yield obtained with the swim-up method (6.4 + 0.8 X 106), which was significantly greater (P < 0.01) than that recovered via the swim-up method. Also, the time required to harvest 'sperm through SpermPrep filtration was 20 to 25 minutes versus 80 minutes required for the swim-up method (P < 0.05). Conclusion: Considering that the effectiveness of frozen-thawed semen is already limited when compared with fresh semen, SpermPrep filtration is the method of choice over the swim-up technique of sperm selection because the former provides significantly greater numbers of high quality sperm. It should be considered as an adjunct in semen preparation for IUI or other forms of assisted reproduction. Fertil Steril 1992;57:1326-30