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Gene of rat Ca 2+ /calmodulin‐dependent protein kinase II α isoform — its cloning and whole structure
Author(s) -
Nishioka Nobuhiro,
Shiojiri Masatoshi,
Kadota Shiori,
Morinaga Hisayo,
Kuwahara Jun,
Arakawa Toshiya,
Yamamoto Shozo,
Yamauchi Takashi
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01105-2
Subject(s) - exon , autophosphorylation , gene isoform , microbiology and biotechnology , biology , splice , intron , splice site mutation , alternative splicing , gene , complementary dna , calmodulin , coding region , rna splicing , protein kinase a , genetics , biochemistry , kinase , rna , enzyme
The gene encoding the α isoform of rat Ca 2+ / calmodulin‐dependent protein kinase II was cloned, and its exon‐intron organization was analyzed. The coding region of cDNA consists of 18 exons spanning more than 50 kilobase pairs. Each of the discrete functional units, such as the ATP‐binding site, the autophosphorylation site responsible for Ca 2+ ‐independent activity, the calmodulin‐binding site, and link structure is encoded by a single exon. The largest and smallest exons consist of 229 and 41 base pairs, respectively. All splice junction sequences flanking the introns conform to the consensus splice junction sequence and the GT‐AG splice rule.