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Expression of chloride channel 1 mRNA in cultured myogenic cells: a marker of myotube maturation
Author(s) -
Bardouille Claudia,
Vullhorst Detlef,
Jockusch Harald
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01098-8
Subject(s) - myogenesis , messenger rna , desmin , skeletal muscle , myocyte , northern blot , microbiology and biotechnology , biology , blot , in situ hybridization , chemistry , medicine , endocrinology , gene , immunohistochemistry , genetics , immunology , vimentin
The chloride channel ClC‐1 is required to maintain a normal excitability of mature muscle fibers; its blockade leads to hyperexcitability, the hallmark of the disease myotonia. In mouse and rat myotubes, representing the embryonic stage of muscle, ClC‐1 mRNA is not detectable by Northern blotting. From neonatal to adult, ClC‐1 expression increases at least fourfold. Using RT‐PCR and hybridization on cultured myotubes were found ClC‐1 mRNA at a level of 0.4–1.1% of that in mature mouse muscle, and ≤0.01% in myoblasts, at stages when desmin mRNA levels are already high. The level of ClC‐1 mRNA is thus a sensitive and specific indicator of the maturation of skeletal muscle cells.