Premium
Inhibitor/fatty acid interactions with cytochrome P‐450 BM3
Author(s) -
Macdonald Iain D.G.,
Ewen Smith W.,
Munro Andrew W.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01095-2
Subject(s) - chemistry , cytochrome , resonance raman spectroscopy , hemeprotein , circular dichroism , substrate (aquarium) , fatty acid , stereochemistry , heme , spectroscopy , ligand (biochemistry) , biochemistry , raman spectroscopy , enzyme , biology , receptor , ecology , physics , quantum mechanics , optics
The interaction of fatty acid substrate (palmitate) and inhibitor (metyrapone: 2‐methyl‐1,2‐di‐3‐pyridyl‐1‐propanone) with cytochrome P‐450 BM3 was analysed by UV‐visible and circular dichroism spectroscopy, and by surface‐enhanced resonance Raman scattering (SERRS). While visible spectroscopy provides information on the relative affinities of these compounds, SERRS provides additional novel data indicating palmitate‐induced structural changes in the haem environment. SERRS also demonstrates that binding of both palmitate and the large nitrogenous ligand metyrapone occurs simultaneously to P‐450 BM3 — highlighting the usefulness of this technique in probing haemoprotein active sites.