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Vitamin D 3 elicits calcium response and activates blood monocyte‐derived macrophages from patients with vitamin D dependent rickets type II
Author(s) -
Takeda Eiji,
Takata Kazumi,
Yamanaka Hisami,
Taketani Yutaka,
Morita Kyoto,
Miyamoto Ken-ichi,
Shono Masayuki,
Teshima Shigetada,
Rokutan Kazuhito
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01093-9
Subject(s) - rickets , vitamin d and neurology , vitamin , calcium , endocrinology , monocyte , medicine , chemistry , intracellular , biochemistry
We studied the effects of vitamin D 3 metabolites on intracellular free Ca 2+ concentration ([Ca 2+ ] i ) and the respiratory burst of monocyte‐derived macrophages (MDM) from patients with vitamin D dependent rickets type II. Treatment of MDM from the patients and healthy donors with 1 nM 1,25(OH) 2 D 3 produced a rapid elevation of [Ca 2+ ] i and similarly primed both types of cells for enhanced capacity for O 2 − release with phorbol diester. These results suggest that macrophages may have distinct non‐genomic pathways of vitamin D 3 , which partly explain the absence of immunodeficiency and the disappearance of rickets after treatment with vitamin D 3 in the patients.