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Acrosome membrane associated protein kinase activity in hamster spermatozoa
Author(s) -
Devi K.Uma,
Ahmad M.Basheer,
Shivaji S.
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01077-0
Subject(s) - casein kinase 2 , protein kinase a , mitogen activated protein kinase kinase , map2k7 , casein kinase 1 , tyrosine kinase , biochemistry , cyclin dependent kinase 2 , cyclin dependent kinase 9 , map kinase kinase kinase , acrosome reaction , chemistry , c raf , hamster , biology , microbiology and biotechnology , phosphorylation , signal transduction , in vitro
Acrosomal membranes isolated from the caput and cauda epididymal spermatozoa of hamster exhibited protein kinase activity and the endogenous protein substrates that were phosphorylated in the acrosomal membranes of caput and cauda spermatozoa were not all the same. The kinase activity was identified as a cAMP independent type and the use of specific stimulators and inhibitors indicated that the activity was not due to casein kinase, protein kinase A or protein kinase C but due to a tyrosine specific protein kinase that was not inhibited by Genistein. Phosphotyrosine was identified as the predominant phosphorylated residue in the proteins.