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Identification and characterization of a lower plant Ypt/Rab guanosine dissociation inhibitor (GDI)
Author(s) -
Beyser Kurt,
Fabry Stefan
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01068-x
Subject(s) - rab , chemistry , guanosine , guanosine diphosphate , biochemistry , gtpase , gtp' , guanosine triphosphate , enzyme
The cDNA encoding a Ypt/Rab guanosine dissociation inhibitor (Ypt‐GDI) was isolated from the multicellular green alga Volvox carteri , representing the first complete plant gdi gene described. The gdiV1 gene occurs as a single copy in the algal genome, indicating that its product regulates all YptV proteins from Volvox . The derived GDI protein (GDIV1p) shows high similarity to animal and fungal GDIs. A specific antibody developed against GDIV1p detected the protein throughout the whole Volvox life‐cycle. GDIV1p was localized in the cytoplasm and in the algal flagellum. This is in line with earlier findings of a dual localization of Ypt proteins both in the cell body and in the motility organelle, and indicates a novel role of the GDI/Ypt system, possibly in intraflagellar transport.