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Folding and aggregation of DNA chains induced by complexation with lipospermine: formation of a nucleosome‐like structure and network assembly
Author(s) -
Yoshikawa Yuko,
Emi Nobuhiko,
Kanbe Toshio,
Yoshikawa Kenichi,
Saito Hidehiko
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01057-5
Subject(s) - dna , biophysics , nucleosome , folding (dsp implementation) , transfection , cationic polymerization , chemistry , molecule , dna origami , microbiology and biotechnology , crystallography , histone , biology , gene , biochemistry , organic chemistry , electrical engineering , engineering
Dioctadecylamidoglycylspermine (DOGS) is a cationic lipid vector capable of efficiently introducing DNA into various eukaryotic cells. We investigated the higher‐order structure of the DNA/DOGS complex using fluorescence and electron microscopy. Our results show that the DNA/DOGS complex exhibits a nucleosome‐like structure in which DNA wraps around an aggregate of DOGS molecules. In addition, DNA/DOGS complexes tend to associate with each other to form network structures. The resulting network assembly may play a role in effective gene transfection.