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The activity of the gluconate‐H + symporter of Schizosaccharomyces pombe cells is down‐regulated by d ‐glucose and exogenous cAMP
Author(s) -
Caspari Thomas,
Urlinger Stefanie
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01052-6
Subject(s) - schizosaccharomyces pombe , symporter , glycerol kinase , snf3 , glucose transporter , extracellular , biochemistry , glycerol , chemistry , schizosaccharomyces , kinase , yeast , glucose uptake , biology , transporter , insulin , saccharomyces cerevisiae , endocrinology , gene
Schizosaccharomyces pombe cells take up d ‐gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol‐containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of d ‐glucose as well as its non‐metabolizable analogues to glycerol‐grown cells causes an immediate loss of gluconate transport within 1 min. The reversible down‐regulation of the gluconate carrier occurs after glucose has been internalized. This regulation is triggered not only by d ‐glucose but also by extracellular cAMP even in the absence of the cAMP‐dependent protein kinase (PKA1).