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Functional conservation of mouse Notch receptor family members
Author(s) -
Kato Hisamune,
Sakai Takashi,
Tamura Kumiko,
Minoguchi Shigeru,
Shirayoshi Yasuaki,
Hamada Yoshio,
Tsujimoto Yoshihide,
Honjo Tasuku
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01046-0
Subject(s) - notch signaling pathway , biology , fusion protein , microbiology and biotechnology , function (biology) , receptor , intracellular , virus , signal transduction , genetics , gene , recombinant dna
All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP‐Jκ. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP‐Jκ by the GST‐fusion pull down assay and dominant negative competition with Epstein Barr virus nuclear antigen 2. Furthermore the intracellular region of int3 was shown to transactivate the Epstein Barr virus TP1 promoter. These results indicate that all mouse Notch family members have biochemical functions similar to mNotch1, which transduces proliferative signal by direct interaction with the DNA binding protein RBP‐Jκ.