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Tissue‐specific alternative splicing of mouse brain type ryanodine receptor/calcium release channel mRNA
Author(s) -
Miyatake Ryosuke,
Furukawa Aizo,
Matsushita Masayuki,
Iwahashi Kazuhiko,
Nakamura Kazuhiko,
Ichikawa Yoshiyuki,
Suwaki Hiroshi
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01022-8
Subject(s) - rna splicing , alternative splicing , biology , messenger rna , ryanodine receptor , microbiology and biotechnology , splice , receptor , gene , rna , genetics
We detected alternative splicing of the mouse brain type ryanodine receptor (RyR3) mRNA. The splicing variant was located in the transmembrane segment. The non‐splicing type (RyR3‐II) included a stretch of 341 bp, and that of the 13th codon was stop codon TAA. Reverse transcription‐polymerase chain reaction (RT‐PCR) analysis shows that RyR3‐II mRNA was expressed in various peripheral tissues and brain at all developmental stages. However, interestingly, the splicing type (RyR3‐I) mRNA was detected only in the cerebrum. These findings suggest that the splicing variants RyR3‐I and RyR3‐II may generate functional differences of RyR3 in a tissue‐specific manner.