Premium
Lipoxygenase activity in heart cells
Author(s) -
Breitbart Eyal,
Sofer Yossi,
Shainberg Asher,
Grossman Shlomo
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01017-4
Subject(s) - arachidonic acid , microsome , lipoxygenase , radioimmunoassay , enzyme , chemistry , biochemistry , leukotriene , chromatography , metabolism , thin layer chromatography , microbiology and biotechnology , biology , immunology , asthma
Arachidonic acid (AA) metabolism via the lipoxygenase (LOX) pathway in rat hearts and in cultured rat cardiomyocytes was investigated using 1‐[ 14 C]AA. LOX activity was detected in the microsomal fraction, in the high speed supernatant prepared from rat hearts and in rat cardiomyocyte supernatant. LOX products from all fractions comigrated in thin layer chromatography as 12‐hydroxyeicosatetraenoic acid (12‐HETE) and 15‐HETE. Enzyme linked immunosorbent assay for 12‐HETE showed its formation by the microsomal fraction, the ammonium sulfate (AS) pellet, and by rat cardiomyocyte supernatant, while radioimmunoassay for 15‐HETE showed its formation only by the AS pellet. The properties of LOX in each fraction are reported here.