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Expression of photoreceptor cyclic nucleotide‐gated cation channel α subunit (CNGCα) in the liver and skeletal muscle
Author(s) -
Feng Li,
Subbaraya Iswari,
Yamamoto Nair,
Baehr Wolfgang,
Kraus-Friedmann Naomi
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01011-3
Subject(s) - skeletal muscle , intracellular , protein subunit , endocrinology , medicine , nucleotide , glucagon , cyclic nucleotide , chemistry , biology , ion channel , hormone , biochemistry , gene , receptor
Glucagon and β‐adrenergic agents increase cAMP levels and stimulate Ca 2+ influx in liver cells. There is no consensus as to the mechanism by which these hormones stimulate the influx of Ca 2+ . Using mouse retinal rod CNGCα cDNA probes, we cloned rat liver and skeletal muscle, and human hepatic CNGCα subunit sequences showing 97–100% identity with the human rod channel. In order to assess channel activity, the effect of cyclic nucleotides on free intracellular Ca 2+ levels of isolated hepatocytes was measured. Dibutyryl‐cAMP was more effective in increasing free Ca 2+ levels than dibutyryl‐cGMP. These data indicate that the CNGCα subunit is expressed in the both liver and skeletal muscle possibly mediating hormonal effects on ion fluxes.