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Expression of the gene for tear lipocalin/von Ebner's gland protein in human prostate
Author(s) -
Holzfeind Paul,
Merschak Petra,
Rogatsch Hermann,
Culig Zoran,
Feichtinger Hans,
Klocker Helmut,
Redl Bernhard
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01008-3
Subject(s) - complementary dna , microbiology and biotechnology , biology , lipocalin , prostate , messenger rna , cdna library , blot , gene expression , gene , endocrinology , biochemistry , cancer , genetics
Northern analysis of human multiple tissue blots containing poly A + RNA from spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes revealed that a prostate specific transcript hybridizes to a tear lipocalin/von Ebner's gland protein (TL/VEGP) gene probe. To characterize this transcript, the corresponding cDNA was amplified by reverse transcription (RT)‐PCR. Cloning and sequence analysis showed that it was identical to the tear lipocalin cDNA isolated from human lachrymal glands. Immunohistochemical analysis on thin layer sections of human prostate using a tear lipocalin specific antiserum confirmed the expression of this cDNA in prostate. Thus, our results clearly argue against a unique function of TL/VEGP in human tear fluid or saliva. The human cDNA was expressed in E. coli using the pQE system yielding a recombinant protein which shows biochemical properties identical to the native TL/VEGP.