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Dictyostelium cytosolic fucosyltransferase synthesizes H type 1 trisaccharide in vitro
Author(s) -
Trinchera Marco,
Bozzaro Salvatore
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)01003-4
Subject(s) - dictyostelium , trisaccharide , fucosyltransferase , chemistry , cytosol , in vitro , microbiology and biotechnology , biochemistry , biology , enzyme , gene
A fucosyltransferase activity has been detected using lacto‐N‐biose I as acceptor in the lower eukaryote Dictyostelium discoideum . This transferase requires divalent cations and is inhibited by N ‐ethylmaleimide and detergent treatment. Apparent calculated K m values for GDP‐Fuc and lacto‐N‐biose I are 1.27 μM and 2.80 mM, respectively. The activity is quantitatively recovered in the supernatant after centrifugation at 100 000 × g for 1 h. The reaction product, as determined by gel permeation chromatography, sensitivity to fucosidases, and analysis of partially methylated derivatives, is Fucα1‐2Galβ1‐3GlcNAc (H type 1 trisaccharide).