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Hypoosmolarity and glutamine increased the β‐actin gene transcription in isolated rat hepatocytes
Author(s) -
Husson Annie,
Quillard Muriel,
Fairand Alain,
Chedeville Arlette,
Lavoinne Alain
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00985-4
Subject(s) - transcription (linguistics) , gene expression , microbiology and biotechnology , northern blot , actin , gene , messenger rna , glutamine , chemistry , stimulation , biology , biochemistry , endocrinology , amino acid , philosophy , linguistics
The mechanism of action of hydration state was studied on β‐actin gene expression in isolated hepatocytes. Results obtained with Northern blot analysis and run on transcription assays show that hypoosmolarity increased and hyperosmolarity decreased the β‐actin mRNA level through a corresponding modulation of the rate of the gene transcription. Glutamine, which is known to induce cell swelling, also increased the β‐actin mRNA level in a dose‐dependent manner and induced a stimulation of the β‐actin gene transcription. Thus, cell hydration state regulates gene expression in the liver through a transcriptional mechanism.