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Molecular cloning of frog secretogranin II reveals the occurrence of several highly conserved potential regulatory peptides
Author(s) -
Anouar Youssef,
Jégou Sylvie,
Alexandre David,
Lihrmann Isabelle,
Michael Conlon J.,
Vaudry Hubert
Publication year - 1996
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(96)00976-3
Subject(s) - biology , complementary dna , peptide sequence , amino acid , microbiology and biotechnology , conserved sequence , biochemistry , gene
Secretogranin II (SgII) is an acidic secretory protein present in large dense core vesicles of neuronal and endocrine cells. Based on the sequence of a peptide derived from the processing of SgII in the brain of the frog Rana ridibunda , degenerate oligonucleotides were used to clone the cDNA encoding frog SgII from a pituitary cDNA library. This cDNA encodes a 574 amino acid protein which exhibits 46–48% sequence identity with mammalian SgII and contains 11 pairs of basic amino acids. Four potential processing products delimited by pairs of basic residues exhibited a much higher degree of identity (68–82%) with the corresponding mammalian SgII sequences. The frog SgII mRNA is ∼4 kb in length and is differentially expressed in the brain and endocrine tissues. The present data reveal that several SgII‐derived peptides have been highly conserved during evolution, suggesting that these peptides may play important neuroendocrine regulatory functions.